Coding

Part:BBa_K3505023:Design

Designed by: Asteria Tsapadikou,Emmanouil Stylianakis   Group: iGEM20_Thessaly   (2020-10-20)


FFAR2:V2tail:TCS GB compatible with B2-B3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 106
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 284
    Illegal PstI site found at 864
    Illegal NgoMIV site found at 364
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 560
    Illegal SapI site found at 815
    Illegal SapI.rc site found at 17


Design Notes

gb


Source

source

References